TY - JOUR
T1 - Comparison of ceftazidime-avibactam susceptibility testing methods against OXA-48-like carrying Klebsiella blood stream isolates
AU - Isler, Burcu
AU - Vatansever, Cansel
AU - Özer, Berna
AU - Çınar, Güle
AU - Aslan, Abdullah Tarık
AU - Stewart, Adam
AU - Simos, Peter
AU - Falconer, Caitlin
AU - Bauer, Michelle J.
AU - Forde, Brian
AU - Harris, Patrick
AU - Şimşek, Funda
AU - Tülek, Necla
AU - Demirkaya, Hamiyet
AU - Menekşe, Şirin
AU - Akalin, Halis
AU - Balkan, İlker İnanç
AU - Aydın, Mehtap
AU - Tigen, Elif Tükenmez
AU - Demir, Safiye Koçulu
AU - Kapmaz, Mahir
AU - Keske, Şiran
AU - Doğan, Özlem
AU - Arabacı, Çiğdem
AU - Yağcı, Serap
AU - Hazırolan, Gülşen
AU - Bakır, Veli Oğuzalp
AU - Gönen, Mehmet
AU - Saltoğlu, Neşe
AU - Azap, Alpay
AU - Azap, Özlem
AU - Akova, Murat
AU - Ergönül, Önder
AU - Paterson, David L.
AU - Can, Füsun
N1 - Publisher Copyright:
© 2022 Elsevier Inc.
PY - 2022/9
Y1 - 2022/9
N2 - Ceftazidime-avibactam exhibits good in vitro activity against carbapenem resistant Klebsiella carrying OXA-48-like enzymes. We tested two hundred unique carbapenem resistant Klebsiella blood stream isolates (71% with single OXA-48-like carbapenemases, including OXA-48, n = 62; OXA-232, n = 57; OXA-244, n = 17; OXA-181, n = 5) that were collected as part of a multicentre study against ceftazidime-avibactam using Etest (bioMérieux, Marcyl’Étoile, France), 10/4 μg disc (Thermo Fisher) and Sensititre Gram Negative EURGNCOL Plates (Lyophilized panels, Sensititre, Thermo Fisher) with the aim of comparing the performances of the Etest and disc to that of Sensititre. Ceftazidime-avibactam MIC50/90 was 2/>16 mg/L for the entire collection and was 2/4 mg/L for single OXA-48-like producers. Categorical and essential agreements between the Etest and Sensititre were 100% and 97%, respectively. Categorical agreement between the disc and Sensititre was 100%. Etest and 10/4 μg discs are suitable alternatives to Sensititre for ceftazidime-avibactam sensitivity testing for OXA-48-like producers.
AB - Ceftazidime-avibactam exhibits good in vitro activity against carbapenem resistant Klebsiella carrying OXA-48-like enzymes. We tested two hundred unique carbapenem resistant Klebsiella blood stream isolates (71% with single OXA-48-like carbapenemases, including OXA-48, n = 62; OXA-232, n = 57; OXA-244, n = 17; OXA-181, n = 5) that were collected as part of a multicentre study against ceftazidime-avibactam using Etest (bioMérieux, Marcyl’Étoile, France), 10/4 μg disc (Thermo Fisher) and Sensititre Gram Negative EURGNCOL Plates (Lyophilized panels, Sensititre, Thermo Fisher) with the aim of comparing the performances of the Etest and disc to that of Sensititre. Ceftazidime-avibactam MIC50/90 was 2/>16 mg/L for the entire collection and was 2/4 mg/L for single OXA-48-like producers. Categorical and essential agreements between the Etest and Sensititre were 100% and 97%, respectively. Categorical agreement between the disc and Sensititre was 100%. Etest and 10/4 μg discs are suitable alternatives to Sensititre for ceftazidime-avibactam sensitivity testing for OXA-48-like producers.
KW - Carbapenem resistant Klebsiella
KW - Ceftazidime-avibactam
KW - Etest
KW - OXA-232
KW - OXA-48-like
KW - Sensititre plate
UR - https://www.scopus.com/pages/publications/85134757073
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=performanshacettepe&SrcAuth=WosAPI&KeyUT=WOS:000831189700002&DestLinkType=FullRecord&DestApp=WOS_CPL
U2 - 10.1016/j.diagmicrobio.2022.115745
DO - 10.1016/j.diagmicrobio.2022.115745
M3 - Article
C2 - 35843111
AN - SCOPUS:85134757073
SN - 0732-8893
VL - 104
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 1
M1 - 115745
ER -
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