Biodegradation of micropollutant naproxen with a selected fungal strain and identification of metabolites

Y. Doruk Aracagök; Hakan Göker; Nilüfer Cihangir

doi:10.1515/znc-2016-0162

Biodegradation of micropollutant naproxen with a selected fungal strain and identification of metabolites

Biyoteknoloji Anabilim Dalı

Ankara University

Araştırma sonucu: Dergiye katkı › Makale › bilirkişi

19 Alıntılar (Scopus)

Özet

Pharmaceuticals are widely used for treating human and animal diseases. Naproxen [(S) 6-methoxy-α-methyl-2-naphthalene acetic acid] and its sodium salt are members of the α-arylpropionic acid group of nonsteroidal anti-inflammatory drugs. Due to excessive usage of naproxen, this drug has been determined even in drinking water. In this study, four fungal strains Phanerochaete chrysosporium, Funalia trogii, Aspergillus Niger, and Yarrowia lipolytica were investigated in terms of naproxen removal abilities. According to LC/MS data, A. Niger was found the most efficient strain with 98% removal rate. Two main by-products of fungal transformation, O-desmethylnaproxen and 7-hydroxynaproxen, were identified by using LC/MS, ¹HNMR, and ¹³CNMR. Our results showed that O-demethylation and hydroxylation of naproxen is catalyzed by cytochrome P450 enzyme system.

Orijinal dil	İngilizce
Sayfa (başlangıç-bitiş)	173-179
Sayfa sayısı	7
Dergi	Zeitschrift fur Naturforschung - Section C Journal of Biosciences
Hacim	72
Basın numarası	5-6
DOI'lar	https://doi.org/10.1515/znc-2016-0162
Yayın durumu	Yayınlandı - 1 May 2017

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10.1515/znc-2016-0162

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title = "Biodegradation of micropollutant naproxen with a selected fungal strain and identification of metabolites",

abstract = "Pharmaceuticals are widely used for treating human and animal diseases. Naproxen [(S) 6-methoxy-α-methyl-2-naphthalene acetic acid] and its sodium salt are members of the α-arylpropionic acid group of nonsteroidal anti-inflammatory drugs. Due to excessive usage of naproxen, this drug has been determined even in drinking water. In this study, four fungal strains Phanerochaete chrysosporium, Funalia trogii, Aspergillus Niger, and Yarrowia lipolytica were investigated in terms of naproxen removal abilities. According to LC/MS data, A. Niger was found the most efficient strain with 98\% removal rate. Two main by-products of fungal transformation, O-desmethylnaproxen and 7-hydroxynaproxen, were identified by using LC/MS, 1HNMR, and 13CNMR. Our results showed that O-demethylation and hydroxylation of naproxen is catalyzed by cytochrome P450 enzyme system.",

keywords = "7-hydroxynaproxen, Aspergillus Niger, O-desmethylnaproxen, biodegradation, fungal cytochrome P450, naproxen",

author = "Aracag{\"o}k, \{Y. Doruk\} and Hakan G{\"o}ker and Nil{\"u}fer Cihangir",

note = "Publisher Copyright: {\textcopyright} 2017 Walter de Gruyter GmbH, Berlin/Boston.",

year = "2017",

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doi = "10.1515/znc-2016-0162",

language = "English",

volume = "72",

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TY - JOUR

T1 - Biodegradation of micropollutant naproxen with a selected fungal strain and identification of metabolites

AU - Aracagök, Y. Doruk

AU - Göker, Hakan

AU - Cihangir, Nilüfer

PY - 2017/5/1

Y1 - 2017/5/1

N2 - Pharmaceuticals are widely used for treating human and animal diseases. Naproxen [(S) 6-methoxy-α-methyl-2-naphthalene acetic acid] and its sodium salt are members of the α-arylpropionic acid group of nonsteroidal anti-inflammatory drugs. Due to excessive usage of naproxen, this drug has been determined even in drinking water. In this study, four fungal strains Phanerochaete chrysosporium, Funalia trogii, Aspergillus Niger, and Yarrowia lipolytica were investigated in terms of naproxen removal abilities. According to LC/MS data, A. Niger was found the most efficient strain with 98% removal rate. Two main by-products of fungal transformation, O-desmethylnaproxen and 7-hydroxynaproxen, were identified by using LC/MS, 1HNMR, and 13CNMR. Our results showed that O-demethylation and hydroxylation of naproxen is catalyzed by cytochrome P450 enzyme system.

AB - Pharmaceuticals are widely used for treating human and animal diseases. Naproxen [(S) 6-methoxy-α-methyl-2-naphthalene acetic acid] and its sodium salt are members of the α-arylpropionic acid group of nonsteroidal anti-inflammatory drugs. Due to excessive usage of naproxen, this drug has been determined even in drinking water. In this study, four fungal strains Phanerochaete chrysosporium, Funalia trogii, Aspergillus Niger, and Yarrowia lipolytica were investigated in terms of naproxen removal abilities. According to LC/MS data, A. Niger was found the most efficient strain with 98% removal rate. Two main by-products of fungal transformation, O-desmethylnaproxen and 7-hydroxynaproxen, were identified by using LC/MS, 1HNMR, and 13CNMR. Our results showed that O-demethylation and hydroxylation of naproxen is catalyzed by cytochrome P450 enzyme system.

KW - 7-hydroxynaproxen

KW - Aspergillus Niger

KW - O-desmethylnaproxen

KW - biodegradation

KW - fungal cytochrome P450

KW - naproxen

UR - https://www.scopus.com/pages/publications/85018399475

U2 - 10.1515/znc-2016-0162

DO - 10.1515/znc-2016-0162

M3 - Article

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AN - SCOPUS:85018399475

SN - 0939-5075

VL - 72

SP - 173

EP - 179

JO - Zeitschrift fur Naturforschung - Section C Journal of Biosciences

JF - Zeitschrift fur Naturforschung - Section C Journal of Biosciences

IS - 5-6

ER -

Biodegradation of micropollutant naproxen with a selected fungal strain and identification of metabolites

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