A kinetic approach to oxidase based enzyme electrodes: The effect of enzyme layer formation on the response time

The formation of the enzyme layer of the sandwich type electrodes has been altered and evaluated in terms of kinetic performance. The detection of H₂O₂ serves to measure the extent of the reaction. The kinetics of the recognition layer are discussed in terms of the response time of the electrode. The enzyme loading between two membranes has altered mass transfer limitations resulting in significant changes in the response of the electrode. The effectiveness factor is used to describe the mass transfer limitations in the system. The response time of the electrode has been increased while the effectiveness factor has been decreased. Steady-state response of the electrodes has been achieved in 43, 68 and 98 s for the electrodes including 23.1%, 13.0% and 7.0% (w/w) GOD in a BSA + GOD mixture, respectively. The effectiveness factors for these electrodes have been established at the values of 0.59, 0.38 and 0.16, respectively.