TY - GEN
T1 - Staphylococcus aureus suşlarinda antibiyotiǧe dirençli gen tanisi için tek kullanimlik MEMS DNA biyosensörü
AU - Ceylan, Hatice
AU - Külah, Haluk
AU - Alp, Aslan
AU - Özgen, Canan
AU - Hasçelik, Gülşen
PY - 2010
Y1 - 2010
N2 - This study presents combination of advantages in MEMS technology and molecular methods for detection of methicillin resistant gene, mecA, in Staphylococcus aureus. A disposable MEMS DNA biosensor was designed and fabricated for optical and electrochemical detections. Adsorption of DNA on gold electrode was examined theoretically and experimentally. In theoretical studies, Lennard Jones potential energies of gold and thiol group, and adsorption kinetics of DNA were discussed and, electrochemical analysis of Hoescht 33258 was given. In experimental studies, 27-mer capture and target probes were used to examine the detection of mecA gene. YOYO®-1-iodide nucleic acid stain was used to check adsorption on disposable gold electrodes while Hoescht 33258 was used as a redox marker to amplify signal in electrochemical detection. Experimental results obtained from fluorescence microscopy demonstrate the applicability of hybridization on gold electrodes. Detection of mecA gene electrochemically needs further study.
AB - This study presents combination of advantages in MEMS technology and molecular methods for detection of methicillin resistant gene, mecA, in Staphylococcus aureus. A disposable MEMS DNA biosensor was designed and fabricated for optical and electrochemical detections. Adsorption of DNA on gold electrode was examined theoretically and experimentally. In theoretical studies, Lennard Jones potential energies of gold and thiol group, and adsorption kinetics of DNA were discussed and, electrochemical analysis of Hoescht 33258 was given. In experimental studies, 27-mer capture and target probes were used to examine the detection of mecA gene. YOYO®-1-iodide nucleic acid stain was used to check adsorption on disposable gold electrodes while Hoescht 33258 was used as a redox marker to amplify signal in electrochemical detection. Experimental results obtained from fluorescence microscopy demonstrate the applicability of hybridization on gold electrodes. Detection of mecA gene electrochemically needs further study.
UR - https://www.scopus.com/pages/publications/77954432131
U2 - 10.1109/BIYOMUT.2010.5479793
DO - 10.1109/BIYOMUT.2010.5479793
M3 - Konferans katkısı
AN - SCOPUS:77954432131
SN - 9781424463824
T3 - 2010 15th National Biomedical Engineering Meeting, BIYOMUT2010
BT - 2010 15th National Biomedical Engineering Meeting, BIYOMUT2010
T2 - 2010 15th National Biomedical Engineering Meeting, BIYOMUT2010
Y2 - 21 April 2010 through 24 April 2010
ER -