Spectral characterization of lysozyme adsorption on dye-affinity beads

Cibacron Blue F3GA-attached magnetic poly(2-hydroxyethyl methacrylate) [mPHEMA] beads were prepared by suspension polymerization of HEMA in the presence of magnetite (Fe₃O₄) nanopowder. Average diameter size of the mPHEMA beads was 150-200 μm. The characteristic functional groups of Cibacron Blue F3GA-attached mPHEMA beads were analyzed by Fourier transform infrared spectrometer (FTIR) and Raman scattering spectrometer. The lysozyme adsorption and desorption characteristics of Cibacron Blue F3GA-attached mPHEMA beads were also investigated using FTIR and Raman spectroscopic techniques. When the Raman spectrum of lysozyme adsorbed mPHEMA is evaluated characteristic Amide-I band appears at 1657 cm^-1. The intensity of this band decreases in the spectrum of lysozyme desorbed mPHEMA sample. When the characteristic bands of lysozyme adsorbed and desorbed mPHEMA samples are compared, the band intensities of desorbed sample are lower than those of lysozyme adsorbed sample except for the band appearing at 656 cm ^-1 (Tyr vC-S).