Separation of Astragalus saponins by reversed phase high performance liquid chromatography and evaporative light scattering detection

M. Ganzera; E. Bedir; I. Çalis; I. A. Khan

doi:10.1007/bf02491559

Separation of Astragalus saponins by reversed phase high performance liquid chromatography and evaporative light scattering detection

M. Ganzera
, E. Bedir
, I. Çalis
, I. A. Khan

Hacettepe University

University of Mississippi

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

A new HPLC method permitted the separation of 13 triterpene glycosides isolated from different Astragalus species within 40 min. A water / acetonitrile gradient was used as eluent and 5 μm RP-18 material as stationary phase. By using an evaporative light scattering (ELS) detector, the main saponins of A. membranaceus could be detected at levels as low as 20.0 μg · mL ^-1. This method facilitated distinction of different Astragalus species as well as the analyses of market products containing A. membranaceus. The results showed variations from 0.019 to 0.184% in the total saponin content of the market products.

Original language	English
Pages (from-to)	131-134
Number of pages	4
Journal	Chromatographia
Volume	53
Issue number	3-4
DOIs	https://doi.org/10.1007/bf02491559
Publication status	Published - 2001

Keywords

Astragalus species
Column liquid chromatography
Evaporative light scattering detection
Saponin

Access to Document

10.1007/bf02491559

Cite this

@article{66083257f5f64d2c9e794cd449f7d924,

title = "Separation of Astragalus saponins by reversed phase high performance liquid chromatography and evaporative light scattering detection",

abstract = "A new HPLC method permitted the separation of 13 triterpene glycosides isolated from different Astragalus species within 40 min. A water / acetonitrile gradient was used as eluent and 5 μm RP-18 material as stationary phase. By using an evaporative light scattering (ELS) detector, the main saponins of A. membranaceus could be detected at levels as low as 20.0 μg · mL -1. This method facilitated distinction of different Astragalus species as well as the analyses of market products containing A. membranaceus. The results showed variations from 0.019 to 0.184\% in the total saponin content of the market products.",

keywords = "Astragalus species, Column liquid chromatography, Evaporative light scattering detection, Saponin",

author = "M. Ganzera and E. Bedir and I. {\c C}alis and Khan, \{I. A.\}",

year = "2001",

doi = "10.1007/bf02491559",

language = "English",

volume = "53",

pages = "131--134",

journal = "Chromatographia",

issn = "0009-5893",

publisher = "Springer",

number = "3-4",

}

TY - JOUR

T1 - Separation of Astragalus saponins by reversed phase high performance liquid chromatography and evaporative light scattering detection

AU - Ganzera, M.

AU - Bedir, E.

AU - Çalis, I.

AU - Khan, I. A.

PY - 2001

Y1 - 2001

N2 - A new HPLC method permitted the separation of 13 triterpene glycosides isolated from different Astragalus species within 40 min. A water / acetonitrile gradient was used as eluent and 5 μm RP-18 material as stationary phase. By using an evaporative light scattering (ELS) detector, the main saponins of A. membranaceus could be detected at levels as low as 20.0 μg · mL -1. This method facilitated distinction of different Astragalus species as well as the analyses of market products containing A. membranaceus. The results showed variations from 0.019 to 0.184% in the total saponin content of the market products.

AB - A new HPLC method permitted the separation of 13 triterpene glycosides isolated from different Astragalus species within 40 min. A water / acetonitrile gradient was used as eluent and 5 μm RP-18 material as stationary phase. By using an evaporative light scattering (ELS) detector, the main saponins of A. membranaceus could be detected at levels as low as 20.0 μg · mL -1. This method facilitated distinction of different Astragalus species as well as the analyses of market products containing A. membranaceus. The results showed variations from 0.019 to 0.184% in the total saponin content of the market products.

KW - Astragalus species

KW - Column liquid chromatography

KW - Evaporative light scattering detection

KW - Saponin

UR - https://www.scopus.com/pages/publications/0035114828

U2 - 10.1007/bf02491559

DO - 10.1007/bf02491559

M3 - Article

AN - SCOPUS:0035114828

SN - 0009-5893

VL - 53

SP - 131

EP - 134

JO - Chromatographia

JF - Chromatographia

IS - 3-4

ER -

Separation of Astragalus saponins by reversed phase high performance liquid chromatography and evaporative light scattering detection

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this