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Interaction of Mitomycin C with DNA Immobilized onto Single-walled Carbon Nanotube/Polymer Modified Pencil Graphite Electrode

  • Ege University

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

The electrochemical investigation of the interaction between the anticancer drug mitomycin C (MC) and DNA was described using a single-walled carbon nanotube (SWCNT)/poly(vinylferrocenium) (PVF+) modified pencil graphite electrode (PGE). The electrochemical oxidation signals of guanine were monitored before and after the interaction between MC and DNA by using differential pulse voltammetry. The effects of DNA and MC concentration and MC interaction time were examined based on the electrode response. Cyclic voltammetry and electrochemical impedance spectroscopy were used for the characterization of SWCNT/PVF+ modified and PVF+ modified PGEs. The detection limit corresponded to 625ng/mL for MC using calf thymus double-stranded DNA immobilized SWCNT/PVF+ modified PGE.

Original languageEnglish
Pages (from-to)2343-2349
Number of pages7
JournalElectroanalysis
Volume23
Issue number10
DOIs
Publication statusPublished - Oct 2011

Keywords

  • DNA
  • Drug-DNA interactions
  • Electroactive polymers
  • Mitomycin C
  • Poly(vinylferrocenium)
  • Single-walled carbon nanotubes

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