TY - JOUR
T1 - Comparative in vitro activity of plazomicin and older aminoglyosides against Enterobacterales isolates; prevalence of aminoglycoside modifying enzymes and 16S rRNA methyltransferases
AU - Plazomicin Study Grp
AU - Gur, Deniz
AU - Hasdemir, Ufuk
AU - Cakar, Asli
AU - Cavusoglu, Iffet
AU - Celik, Tugce
AU - Aksu, Burak
AU - Sancak, Banu
AU - Altun, Belgin
AU - Soyletir, Guner
AU - Ulger, Nurver
AU - Kararuna, Onur
AU - Karahan, Zeynep Ceren
AU - Evren, Ebru
AU - Bakici, Zahir
AU - Celik, Cem
AU - Akpolat, Nezahat
AU - Gulay, Zeynep
AU - Sari, Ayse Nur
AU - Aydemir, Sohret
AU - Cilli, Feriha
AU - Kilic, Huseyin
AU - Parkan, Omiir Mustafa
AU - Zer, Yasemin
AU - Buyuktas, Ayse
AU - Koksal, Fauna
AU - Aydin, Faruk
AU - Ozkaya, Esra
AU - Birinci, Asuman
AU - Cayci, Yeliz Tanriverdi
AU - Tuncer, Inci
PY - 2020/8
Y1 - 2020/8
N2 - Comparative in vitro activity of plazomicin and 4 older aminoglycosides was evaluated with broth microdilution in 714 blood isolates from 14 hospitals in Turkey.Isolates included Escherichia coli (n=320), Klebsiella spp. (n=294), Enterobacter spp. (n =69), Serratia marcescens (n=20). and Citrobacter spp. (n=11). Isolates resistant to older aminoglycosides (n=240) were screened for aminoglycoside modifying enzyme genes: aac(6')-Ib, aac(3)-Ia, aac(3)-IIa, ant(2 '')-Ia. Isolates with high MICs for plazomicin (n=41) were screened for 165 rRNA methyltransferase genes (armA, rmtA, rmtB, rmtC, rmtD, rmtE, rmtF, rmtG, rmtH, npmA) and 2 carbapenemase genes (blaOXA-48, blaNDM-1). Overall, resistance to plazomicin, amikacin, netilmicin, gentamicin, and tobramycin was 7.7%, 7.4%, 31.5%, 32.9%, and 34.7%, respectively. aac(6')-Ib and aac(3)-IIa were the most common AME genes. Co-occurrence of blaNDM-1 with armA and rmtC and blaOXA-48 with armA was striking. Enterobacter cloacae carrying rmtC+blaNDM-1, S. marcescens with armA+blaOXA-48, and rmtF+ blaOXA-48 in K. pneumoniae were reported for the first time. (C) 2020 Elsevier Inc. All rights reserved.
AB - Comparative in vitro activity of plazomicin and 4 older aminoglycosides was evaluated with broth microdilution in 714 blood isolates from 14 hospitals in Turkey.Isolates included Escherichia coli (n=320), Klebsiella spp. (n=294), Enterobacter spp. (n =69), Serratia marcescens (n=20). and Citrobacter spp. (n=11). Isolates resistant to older aminoglycosides (n=240) were screened for aminoglycoside modifying enzyme genes: aac(6')-Ib, aac(3)-Ia, aac(3)-IIa, ant(2 '')-Ia. Isolates with high MICs for plazomicin (n=41) were screened for 165 rRNA methyltransferase genes (armA, rmtA, rmtB, rmtC, rmtD, rmtE, rmtF, rmtG, rmtH, npmA) and 2 carbapenemase genes (blaOXA-48, blaNDM-1). Overall, resistance to plazomicin, amikacin, netilmicin, gentamicin, and tobramycin was 7.7%, 7.4%, 31.5%, 32.9%, and 34.7%, respectively. aac(6')-Ib and aac(3)-IIa were the most common AME genes. Co-occurrence of blaNDM-1 with armA and rmtC and blaOXA-48 with armA was striking. Enterobacter cloacae carrying rmtC+blaNDM-1, S. marcescens with armA+blaOXA-48, and rmtF+ blaOXA-48 in K. pneumoniae were reported for the first time. (C) 2020 Elsevier Inc. All rights reserved.
KW - 16S rRNA methyltransferase
KW - Aminoglycoside modifying enzyme
KW - Aminoglycoside resistance
KW - E. coli
KW - Enterobacter
KW - Klebsiella
KW - Ndm-1
KW - Oxa-48
KW - Plazomicin
KW - Serratia
KW - armA
KW - rmtA
KW - rmtB
KW - rmtC
KW - rmtE
KW - rmtF
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=performanshacettepe&SrcAuth=WosAPI&KeyUT=WOS:000551985300014&DestLinkType=FullRecord&DestApp=WOS_CPL
U2 - 10.1016/j.diagmicrobio.2020.115092
DO - 10.1016/j.diagmicrobio.2020.115092
M3 - Article
C2 - 32569921
SN - 0732-8893
VL - 97
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 4
M1 - 115092
ER -