Cibacron blue F3GA and Cu(II) derived poly (2-hydroxyethylmethacrylate) membranes for lysozyme adsorption

Lysozyme adsorption onto Cibacron Blue F3GA and Cu(II) derived poly(2- hydroxyethyl methacrylate) [poly(HEMA)] membranes was investigated. Microporous poly (HEMA) membranes were prepared by photopolymerization of HEMA. The triazine dye, Cibacron Blue F3GA was attached covalently as a dye- ligand. These dye-membranes with a swelling ratio of 58% (w/w), and carrying different amounts of Cibacron Blue F3GA (between 0.35 and 1.07 μmol cm^-2) were used in the lysozyme adsorption studies. The effect of initial concentration and pH on the adsorption efficiency of dye-derived and metal- chelated membranes were studied in a batch reactor. The effect of Cu(II) incorporation on lysozyme adsorption was also studied. The non-specific adsorption of lysozyme on the poly(HEMA) membranes was 0.9 μg cm^-2. Cibacron Blue F3GA attachment significantly increased the lysozyme adsorption up to 133.3 μg cm^-2. Lysozyme adsorption capacity of the Cu(II) incorporated membranes (165.1 μg cm^-2) was greater than that of the Cibacron Blue F3GA-attached membranes. More than 85% of the adsorbed lysozyme was desorbed in 1 h in the desorption medium containing 0.5 M potassium thiocyanate (KSCN) at pH 8.0.