Cibacron Blue F3G-A Attached Poly(Vinyl Alcohol) Particles for Specific Albumin Adsorption

Adil Denizli; Ali Tuncel; Ahmet Kozluca; Kutay Ecevit; Erhan Pişkin

doi:10.1080/01496399708000942

Cibacron Blue F3G-A Attached Poly(Vinyl Alcohol) Particles for Specific Albumin Adsorption

Adil Denizli
, Ali Tuncel
, Ahmet Kozluca
, Kutay Ecevit
, Erhan Pişkin

Division of Biochemistry

Research output: Contribution to journal › Article › peer-review

47 Citations (Scopus)

Abstract

Poly(vinyl alcohol) (PVAL) particles (average size: 50 μm) were prepared in the present study by chemical crosslinking of PVAL with glutaraldehyde in an organic dispersion oil phase. Cibacron Blue F3G-A was attached to these hydrophilic PVAL particles. Bovine serum albumin (BSA) adsorption onto these dye-attached PVAL particles from aqueous solutions containing different amounts of BSA in three different salts (i.e., NaCl, CaCl₂, and NaSCN) at different pH and ionic strengths was investigated in batch reactors. The maximum adsorption capacity (about 35 mg BSA/g dye-attached PVAL) was observed around pH 6.0 in a medium containing NaCl with an ionic strength of 0.01. Nonspecific BSA adsorption on plain PVAL particles was almost zero. About 90% of the adsorbed BSA was desorbed by using a 0.5 M NaSCN desorption medium for 1 hour.

Original language	English
Pages (from-to)	1003-1015
Number of pages	13
Journal	Separation Science and Technology
Volume	32
Issue number	5
DOIs	https://doi.org/10.1080/01496399708000942
Publication status	Published - 1997

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title = "Cibacron Blue F3G-A Attached Poly(Vinyl Alcohol) Particles for Specific Albumin Adsorption",

abstract = "Poly(vinyl alcohol) (PVAL) particles (average size: 50 μm) were prepared in the present study by chemical crosslinking of PVAL with glutaraldehyde in an organic dispersion oil phase. Cibacron Blue F3G-A was attached to these hydrophilic PVAL particles. Bovine serum albumin (BSA) adsorption onto these dye-attached PVAL particles from aqueous solutions containing different amounts of BSA in three different salts (i.e., NaCl, CaCl2, and NaSCN) at different pH and ionic strengths was investigated in batch reactors. The maximum adsorption capacity (about 35 mg BSA/g dye-attached PVAL) was observed around pH 6.0 in a medium containing NaCl with an ionic strength of 0.01. Nonspecific BSA adsorption on plain PVAL particles was almost zero. About 90\% of the adsorbed BSA was desorbed by using a 0.5 M NaSCN desorption medium for 1 hour.",

author = "Adil Denizli and Ali Tuncel and Ahmet Kozluca and Kutay Ecevit and Erhan Pi{\c s}kin",

year = "1997",

doi = "10.1080/01496399708000942",

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TY - JOUR

T1 - Cibacron Blue F3G-A Attached Poly(Vinyl Alcohol) Particles for Specific Albumin Adsorption

AU - Denizli, Adil

AU - Tuncel, Ali

AU - Kozluca, Ahmet

AU - Ecevit, Kutay

AU - Pişkin, Erhan

PY - 1997

Y1 - 1997

N2 - Poly(vinyl alcohol) (PVAL) particles (average size: 50 μm) were prepared in the present study by chemical crosslinking of PVAL with glutaraldehyde in an organic dispersion oil phase. Cibacron Blue F3G-A was attached to these hydrophilic PVAL particles. Bovine serum albumin (BSA) adsorption onto these dye-attached PVAL particles from aqueous solutions containing different amounts of BSA in three different salts (i.e., NaCl, CaCl2, and NaSCN) at different pH and ionic strengths was investigated in batch reactors. The maximum adsorption capacity (about 35 mg BSA/g dye-attached PVAL) was observed around pH 6.0 in a medium containing NaCl with an ionic strength of 0.01. Nonspecific BSA adsorption on plain PVAL particles was almost zero. About 90% of the adsorbed BSA was desorbed by using a 0.5 M NaSCN desorption medium for 1 hour.

AB - Poly(vinyl alcohol) (PVAL) particles (average size: 50 μm) were prepared in the present study by chemical crosslinking of PVAL with glutaraldehyde in an organic dispersion oil phase. Cibacron Blue F3G-A was attached to these hydrophilic PVAL particles. Bovine serum albumin (BSA) adsorption onto these dye-attached PVAL particles from aqueous solutions containing different amounts of BSA in three different salts (i.e., NaCl, CaCl2, and NaSCN) at different pH and ionic strengths was investigated in batch reactors. The maximum adsorption capacity (about 35 mg BSA/g dye-attached PVAL) was observed around pH 6.0 in a medium containing NaCl with an ionic strength of 0.01. Nonspecific BSA adsorption on plain PVAL particles was almost zero. About 90% of the adsorbed BSA was desorbed by using a 0.5 M NaSCN desorption medium for 1 hour.

UR - https://www.scopus.com/pages/publications/0031094756

U2 - 10.1080/01496399708000942

DO - 10.1080/01496399708000942

M3 - Article

AN - SCOPUS:0031094756

SN - 0149-6395

VL - 32

SP - 1003

EP - 1015

JO - Separation Science and Technology

JF - Separation Science and Technology

IS - 5

ER -

Cibacron Blue F3G-A Attached Poly(Vinyl Alcohol) Particles for Specific Albumin Adsorption

Abstract

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