Biodegradation of micropollutant naproxen with a selected fungal strain and identification of metabolites

Y. Doruk Aracagök; Hakan Göker; Nilüfer Cihangir

doi:10.1515/znc-2016-0162

Biodegradation of micropollutant naproxen with a selected fungal strain and identification of metabolites

Division of Biotechnology

Ankara University

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Pharmaceuticals are widely used for treating human and animal diseases. Naproxen [(S) 6-methoxy-α-methyl-2-naphthalene acetic acid] and its sodium salt are members of the α-arylpropionic acid group of nonsteroidal anti-inflammatory drugs. Due to excessive usage of naproxen, this drug has been determined even in drinking water. In this study, four fungal strains Phanerochaete chrysosporium, Funalia trogii, Aspergillus Niger, and Yarrowia lipolytica were investigated in terms of naproxen removal abilities. According to LC/MS data, A. Niger was found the most efficient strain with 98% removal rate. Two main by-products of fungal transformation, O-desmethylnaproxen and 7-hydroxynaproxen, were identified by using LC/MS, ¹HNMR, and ¹³CNMR. Our results showed that O-demethylation and hydroxylation of naproxen is catalyzed by cytochrome P450 enzyme system.

Original language	English
Pages (from-to)	173-179
Number of pages	7
Journal	Zeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume	72
Issue number	5-6
DOIs	https://doi.org/10.1515/znc-2016-0162
Publication status	Published - 1 May 2017

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

7-hydroxynaproxen
Aspergillus Niger
O-desmethylnaproxen
biodegradation
fungal cytochrome P450
naproxen

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title = "Biodegradation of micropollutant naproxen with a selected fungal strain and identification of metabolites",

abstract = "Pharmaceuticals are widely used for treating human and animal diseases. Naproxen [(S) 6-methoxy-α-methyl-2-naphthalene acetic acid] and its sodium salt are members of the α-arylpropionic acid group of nonsteroidal anti-inflammatory drugs. Due to excessive usage of naproxen, this drug has been determined even in drinking water. In this study, four fungal strains Phanerochaete chrysosporium, Funalia trogii, Aspergillus Niger, and Yarrowia lipolytica were investigated in terms of naproxen removal abilities. According to LC/MS data, A. Niger was found the most efficient strain with 98\% removal rate. Two main by-products of fungal transformation, O-desmethylnaproxen and 7-hydroxynaproxen, were identified by using LC/MS, 1HNMR, and 13CNMR. Our results showed that O-demethylation and hydroxylation of naproxen is catalyzed by cytochrome P450 enzyme system.",

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AU - Aracagök, Y. Doruk

AU - Göker, Hakan

AU - Cihangir, Nilüfer

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Y1 - 2017/5/1

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AB - Pharmaceuticals are widely used for treating human and animal diseases. Naproxen [(S) 6-methoxy-α-methyl-2-naphthalene acetic acid] and its sodium salt are members of the α-arylpropionic acid group of nonsteroidal anti-inflammatory drugs. Due to excessive usage of naproxen, this drug has been determined even in drinking water. In this study, four fungal strains Phanerochaete chrysosporium, Funalia trogii, Aspergillus Niger, and Yarrowia lipolytica were investigated in terms of naproxen removal abilities. According to LC/MS data, A. Niger was found the most efficient strain with 98% removal rate. Two main by-products of fungal transformation, O-desmethylnaproxen and 7-hydroxynaproxen, were identified by using LC/MS, 1HNMR, and 13CNMR. Our results showed that O-demethylation and hydroxylation of naproxen is catalyzed by cytochrome P450 enzyme system.

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KW - Aspergillus Niger

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KW - biodegradation

KW - fungal cytochrome P450

KW - naproxen

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ER -

Biodegradation of micropollutant naproxen with a selected fungal strain and identification of metabolites

Abstract

UN SDGs

Keywords

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