A safer and relatively shorter method for Southern blot hybridization analysis

Hatice Mergen; Ceren Acar; Esra Birben; Reyhan Öner; Cihan Öner

doi:10.1016/S0165-022X(00)00068-3

A safer and relatively shorter method for Southern blot hybridization analysis

Hatice Mergen
, Ceren Acar
, Esra Birben
, Reyhan Öner
, Cihan Öner

Division of Molecular Biology

Hacettepe University

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The study presented here describes a Southern blot hybridization technique which is performed in a shorter time and safer than the classical one. In this study DNA was isolated from a small amount of blood sample and the probe for hybridization was prepared with ³⁵S by polymerase chain reaction. Copyright (C) 2000 Elsevier Science B.V.

Original language	English
Pages (from-to)	73-76
Number of pages	4
Journal	Journal of Biochemical and Biophysical Methods
Volume	44
Issue number	1-2
DOIs	https://doi.org/10.1016/S0165-022X(00)00068-3
Publication status	Published - 10 Jul 2000

Keywords

Polymerase chain reaction (PCR)
Radioactive labelling
Southern blotting

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10.1016/S0165-022X(00)00068-3

Cite this

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title = "A safer and relatively shorter method for Southern blot hybridization analysis",

abstract = "The study presented here describes a Southern blot hybridization technique which is performed in a shorter time and safer than the classical one. In this study DNA was isolated from a small amount of blood sample and the probe for hybridization was prepared with 35S by polymerase chain reaction. Copyright (C) 2000 Elsevier Science B.V.",

keywords = "Polymerase chain reaction (PCR), Radioactive labelling, Southern blotting",

author = "Hatice Mergen and Ceren Acar and Esra Birben and Reyhan {\"O}ner and Cihan {\"O}ner",

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T1 - A safer and relatively shorter method for Southern blot hybridization analysis

AU - Mergen, Hatice

AU - Acar, Ceren

AU - Birben, Esra

AU - Öner, Reyhan

AU - Öner, Cihan

PY - 2000/7/10

Y1 - 2000/7/10

N2 - The study presented here describes a Southern blot hybridization technique which is performed in a shorter time and safer than the classical one. In this study DNA was isolated from a small amount of blood sample and the probe for hybridization was prepared with 35S by polymerase chain reaction. Copyright (C) 2000 Elsevier Science B.V.

AB - The study presented here describes a Southern blot hybridization technique which is performed in a shorter time and safer than the classical one. In this study DNA was isolated from a small amount of blood sample and the probe for hybridization was prepared with 35S by polymerase chain reaction. Copyright (C) 2000 Elsevier Science B.V.

KW - Polymerase chain reaction (PCR)

KW - Radioactive labelling

KW - Southern blotting

UR - https://www.scopus.com/pages/publications/0034632251

U2 - 10.1016/S0165-022X(00)00068-3

DO - 10.1016/S0165-022X(00)00068-3

M3 - Article

C2 - 10889277

AN - SCOPUS:0034632251

SN - 0165-022X

VL - 44

SP - 73

EP - 76

JO - Journal of Biochemical and Biophysical Methods

JF - Journal of Biochemical and Biophysical Methods

IS - 1-2

ER -

A safer and relatively shorter method for Southern blot hybridization analysis

Abstract

Keywords

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