5-FU'NUN INTRAPERITONEAL KULLANIMINDA INTESTINAL ANASTOMOZ UZERINE OLAN OLUMSUZ ETKISINIIN CINKO ILE ENGELLENMESI

Today adjuvant therapy using early postoperative intraperitoneal chemotherapy, is particularly appropriate treatment to prevent the local and regional recurrence in resectable colon cancers. Intraperitoneal 5-Fluorouracil (5-FU) is the most preferable agent for this purpose. The aim of this study is to determine the effect of Zn against the inhibitory effect of 5-FU on the healing of colonic anastomosis. In 5-FU treated group, average bursting pressure (p:0.048) and hydroxyproline levels were significantly decreased (p:0.015). In only Zn treated group, average bursting pressure was significantly increased (p:0.02) whereas hydroxyproline levels showed no correlation with the control group (p:0.560). In both 5-FU and Zn treated groups average bursting pressure had statistically significant correlation only with the 5-FU treated group (p:0.014). In this group hydroxyproline levels were increased as well (p:0.014). The histological observations showed that 5-FU impaired the healing of colonic anastomosis with the appearance of necrotic tissue at the anastomosis region. However the 5-FU and Zn groups appeared to be nearly completely epithelialized and also the number of fibroblasts were increased while necrotic tissue was not as much as in the 5-FU treated group. We conclude that Zn addition modulates healing of colonic anastomosis by counteracting the negative effect of 5-FU.